Isolation of 2-phenylethanol biosynthesis related gene and developmental patterns of emission of scent compounds in Persian musk rose (Rosa moschata Herrm.)

Abstract

Abstract The molecular properties of 2-phenylethanol were investigated in the single petals of Persian Musk rose at four floral developmental stages. The chemical compositions of the floral scent emissions were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In this plant, the relative percentage of 2-phenylethanol increased as flowers developed. In the early stage of flower development, the patterns of emission of 2-phenylethanol were very low and then progressively increased reaching its highest value at full bloom stage. The partial CDS of phenyl acetaldehyde reductase (PAR) were isolated in the petals of Persian musk rose. Moreover, the expression pattern of RmPAR showed a significant difference during flower developmental stages and floral organ parts of Rosa moschata. The expression level of RmPAR constantly decreased from the budding stage (stage 1) to the full bloom stage (stage 3); It is reached at the highest level in the budding stage and rapidly decreases in the full bloom stage, and then increases again in the senescence stage (stage 4). In addition, the highest expression level of RmPAR was in the stamen and slightly higher in the petal, and the expression amount in the pistil was about half that in the stamen. Nevertheless, in the calyxes, RmPAR was lowly expressed.