Abstract
Abstract. Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.
Highlights
Development and application of genetic markers in turkey (Meleagris gallopavo) began in the mid 1990’s (Huang et al 1999, Levin et al 1995, Smith et al 1996b)
At the same time avian genomes were reported to contain a lower number of microsatellites, compared to mammals (Primmer et al 1997) seemingly complicating the discovery of turkey-specific markers
We report the isolation of 52 dinucleotide sequences from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) with the characterisation and mapping of eleven new loci
Summary
Development and application of genetic markers in turkey (Meleagris gallopavo) began in the mid 1990’s (Huang et al 1999, Levin et al 1995, Smith et al 1996b). Different methods of random genome analyses such as randomly amplified polymorphic DNA (RAPD), single primer amplification of simple sequence repeats (SPARS), and application of chicken and turkey microsatellites were used to estimate genetic diversity within the species (Smith et al 1996a).
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