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Abstract
A class III chitinase gene, Chi3-P1, was cloned from pepper ( Capsicum annuum L.) by sequence-based PCR walking with degenerate primers. According to the DNA sequence, Chi3-P1 encodes a chitinase of 295 amino acid residues with a molecular mass of 32 kDa. It shows sequence similarities of 62 and 57% on the DNA and amino acid levels, respectively, to the tobacco clone P29060 ( Nicotiana tabacum). Neighbor–Joining analysis showed a close phylogenetic relationship of Chi3-P1 to the sub-branch of class III chitinase of Arabidopsis thaliana (P19172). DNA sequences of Chi3-P1 were compared between five pepper inbred lines, H3, Vania, Perennial, Yolo Wonder and Criollo de Morelos 334. The alignment revealed single nucleotide polymorphisms in the protein-coding region. The location of Chi3-P1 in the pepper genome was determined by genetic linkage analysis with RFLP and CAPS.
Published Version
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