Isolation, identification & production of bioactive metabolites from marine fungi collected from coastal area of Andhra Pradesh, India

Abstract

AimTo explore antibacterial active compounds from Curvularia sp. and Aspergillus sp. isolated from marine source. MethodTo increase the bioactive metabolite production and to search novel compound, in my on-going research we collected the source sample from marine water. By the serial dilution method, different dilutions of sample are spread on Rose Bengal medium to isolate pure colonies. Further characterization is done by the microscopic and macroscopic studies. Based on morphological and microscopic studies organisms were identified as Curvularia species, and Aspergillus species. These fungal cultures were grown in potato dextrose agar for 5–6 days for metabolite production. Crude metabolite was extracted using Rota vapor. Metabolite is tested for biological activities and further step TLC was performed to investigate the number of fractions present in crude extract. ResultsMarine fungi organisms were isolated and screened by serial dilution method from Rose Bengal medium. Pure colony was isolated and identified as Curvularia sp. and Aspergillus sp. by slide culture technique. Individual organisms were inoculated in potato dextrose broth for production of secondary metabolites for a period of 6–8 days. The collected crude extract of Curvularia sp. showed 27 mm diameter zone against Enterococcus faecalis and Bacillus megaterium at 100 μg and Aspergillus sp. showed 12 mm diameter zone and best activity against B. megaterium and Xanthomonas campestris at 40 μg. TLC of Curvularia sp. crude extract showed 2 fractions and Aspergillus sp. crude extract showed 5 fractions. ConclusionHence we conclude that the compound has high efficiency of antibacterial activity, which needs extensive attention in terms of new therapy.