Isolation, identification and toxicity of three strains of Heterocapsa (Dinophyceae) in a harmful event in Fujian, China

Abstract

Heterocapsa is a genus of dinoflagellates including species that can form harmful algae blooms (HABs) and cause deleterious ecological effects. To date two species ( H circularisquama and H bohaiensis) are known to be toxic. Here we isolated three strains of Heterocapsa from a multi-species dinoflagellate bloom in the aquaculture area of Fujian, China in June, 2019 that caused mass mortality of farmed abalone. Morphological analysis using light, transmission electron and scanning electron microscopy along with phylogenetic analyses with small (SSU) and large (LSU) subunit rRNA and internal transcribed spacer (ITS) gene sequences showed that these strains were H. cf. niei H. horiguchii, and H. cf. pygmaea, respectively. Furthermore, rabbit erythrocyte assay revealed hemolytic activity in all three strains in cell density dependent fashion, and only in the presence of light. In addition, the strains caused significant mortality of Artemia salina, and the toxicity was also cell density dependent. The Heterocapsa cultures and toxicity information obtained in this study expanded our knowledge of toxic species of Heterocapsa, and will facilitate further investigating the mechanism of their toxicity and developing monitoring tools for their blooms in the future.