Isolation, identification and screening of hidrolytic enzymes producing phylloplane yeasts

Gilberto De Aguiar Pereira,Fernando Gomes Barcellos,Guilherme Teixeira Gomes,Amanda Machado Rozolem,Elisete Pains Rodrigues,Gisele Maria De Andrade De Nóbrega

doi:10.1186/1753-6561-8-s4-p261

Gilberto De Aguiar Pereira, Fernando Gomes Barcellos + Show 4 more

Open Access

https://doi.org/10.1186/1753-6561-8-s4-p261

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Journal: BMC Proceedings	Publication Date: Oct 1, 2014
Citations: 6	License type: cc-by

Affiliation: Universidade Estadual de Londrina

Abstract

Background Microorganisms are common colonizers of superficial (phylloplane) and internal tissues (endophyte) of a variety plant species [1]. Phylloplane yeasts are considered a promising source of new and interesting biotechnological activities, particularly hydrolytic enzymes; however, the diversity of yeasts colonizing plant phylloplane and is still poorly known for most plant species. Considering the immense diversity of Brazilian flora, which include many species with medicinal properties, this knowledge is even more limited. Given the above, this study objective to isolate, identify and to evaluate the production of hidrolytic enzymes of yeasts associated with leaves, stems and flowers of alecrim-do-campo (Baccharis dracunculifolia DC), a plant recognized as the main source of exudates used by bees to produce green propolis and also, for its medicinal properties.

Highlights

Microorganisms are common colonizers of superficial and internal tissues of a variety plant species [1]
On the basis of on colony morphology the isolates were grouped into four morphotypes
By analysis of ITS1-5,8S-ITS2 rDNA sequences in Genbank database, the isolates were identified as members of the species Starmerella bombicola, Aureobasidium leucospermi, A. pullulans, Rhodotorulla mucilaginosa and Occultifur externus

Summary

Introduction

Microorganisms are common colonizers of superficial (phylloplane) and internal tissues (endophyte) of a variety plant species [1]. Methods For isolation of yeasts, samples leaves, stems and flowers of B. dracunculifolia were colected; 10 g were macered in 90 mL of saline solution (0,85%) and dilutions of the suspension were spread in solid medium BDA and TSA. The isolates were characterized by colony morphology and grouped. Isolates representant of each group were subsequently identified by molecular techniques based on the amplification and sequence analysis of ITS1-5, 8S-ITS2 rDNA.

Results

Conclusion