Abstract
Rhizobium, a nitrogen-fixing bacteria is the essential feature of leguminous plants which is essential for the regeneration of nutrient-deficient soil. This study was aimed to isolate, identify, mass culture and immobilize Bradyrhizoium japonicum in encapsulated form and test their viability. Root nodules were sterilized, grinded and cultured aseptically in YEMA media containing Congo red. The obtained colon was sub-cultured to get a pure culture and different biochemical tests were conducted which proved Bradyrhizobium japonicum as the slow-growing species. The test shows a positive result of catalase production and nodulation test whereas the pH tolerance test shows more tolerance to the acidic pH. Similarly, Bradyrhizaobium japonicum can tolerate 1% and 2% NaCl concentration and it doesn’t show resistance to the penicillin disc of 10mg. The mass culture and encapsulation with sodium alginate adding sucrose as nutrient proved the simplicity for handling. Altogether 548 beads were prepared from the 100ml of the cultured broths which were viable for more than 190 days at 1%, 2% and 3% sucrose concentration but less viable at 5% and 10% sucrose concentration under room temperature.
Highlights
A distinctive characteristic of the majority of legumes is their ability to enter into a nitrogenfixing symbiosis with a distinct group of soil bacteria collectively called root nodules bacteria or the Rhizobia [1,2]
Present study was carried on Bradyrhizobium japonicum and it was based on the Rhizobium present on the root nodules of the soybean species found in Nepal
The Rhizobial isolates of the present study shows the less resistance to the penicillin disc 10μg which indicate that penicillin is effective to the Bradyrhizobium japonicum which reduced the growth of the rhizobium showing the antimicrobial activity to rhizobia
Summary
A distinctive characteristic of the majority of legumes is their ability to enter into a nitrogenfixing symbiosis with a distinct group of soil bacteria collectively called root nodules bacteria or the Rhizobia [1,2]. The productivity and sustainability of agriculture throughout the globe are being significantly enhanced through nitrogen fixation from effectively nodulated legumes [3]. Apart from direct benefit from effective nitrogen fixation [4] legumes and Rhizobium provides added value in weed, pathogen and insect control when rotated with crop in farming system [5] together with improving soil structure and increasing soil organic matter content [6]. The encapsulation of the inoculants with polyacrylamide maintained the suitable moisture content. These formulations of immobilized cells protect the microorganism against the environmental stresses and release them to the soil gradually when the polymers are degraded [8]. Increasing the efficiency of the use of available soil nitrogen can meet the additional Nitrogen demand by making cereal plants capable of fixing its own nitrogen through close
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