Abstract
The morphology and the drug sensitivity of the strain GYX2014-1 isolated from the hepatic pancreatic tissue of moribund Litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. Detection of extracellulase and hemolysin activity shows that the isolated GYX2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. The 16S rRNA gene (GenBank accession number: KT781675) was analyzed, and a phylogenetic tree analysis showed that the isolated pathogen was most closely related to V. vulnificus (GenBank accession number: NR 118570)—a match of more than 99%. The phenotypic traits and molecular biology of isolated bacteria, determined their identity as Vibrio vulnificus (V. vulnificus). In addition, artificially infected L. vannamei with Vibrio vulnificus appeared with the same disease symptoms as those of naturally infected shrimp. Drug sensitivity tests showed that V. vulnificus is highly sensitive to fosfomycin, cefradine and sinomin, and was resistant to penicillin, amikacin and kanamycin. This experiment is the first to separate V. vulnificus from L. vannamei, and the findings of this study can be used as a reference for disease control and health management.
Highlights
Penaeus vannamei Boone, scientific name Litopenaeus vannamei [1], commonly known as white-leg shrimp or white shrimp, are eurythermal euryhalinous tropical shrimp
We studied the phenotypic characteristics of the isolated bacteria, their main biological characteristics, extracellular enzymes produced, their 16S rRNA gene sequence and conducted phylogenetic analysis which detected the sensitivity of the bacteria to antibacterial drugs in order to provide a reference point for further research on effective inspection of L. vannamei for Vibrio and basis for prevention and control of disease epidemiology
Bacteria of Vibrios spp. are ubiquitous and the Vibrio genus contains numerous kinds of species relevant to food safety [28]—their presence which pose a big threat to public health
Summary
Penaeus vannamei Boone, scientific name Litopenaeus vannamei [1], commonly known as white-leg shrimp or white shrimp, are eurythermal euryhalinous tropical shrimp. Their appearance resembles Chinese shrimp, Penaeus merguiensis. Their body length measures up to 24 cm and they have a thin, light gray in color shell with no body markings. Since 1994–1995, L. vannamei have been successfully artificially bred in China. Because of their rarity in naturally occurring environments they are widely popular among Asian shrimp producers—accounting for more than
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