Isolation, Identification and Molecular Characterization of Indigenous Bacterial Isolates from Textile Effluent and Contaminated Soil using 16s rRNA Sequencing

Abstract

Textile industry effluent poses a serious environmental threat. Many physical and chemical methods for treating wastewater have been used, but due to high operating costs, a suitable alternative is required. As a result, the study focuses on the use of biological organisms with bioremediation potential which leads to the development of cost-effective and nontoxic methods. The present study focuses on the isolation of possible bacterial isolates from textile effluent contaminated soil and effluent. A total of four bacterial isolates were successfully isolated, three from textile industry effluent contaminated soil and one from textile industry effluent. Based on the results of morphological, biochemical and molecular datasets, the current study identified bacterial isolates belonging to three genera: Brevibacillus, Achromobacter and Pseudomonas. The 16S rRNA sequence technique was used to characterize and identify the bacterial isolates. The isolates were submitted to GenBank like Achromobacter xylosoxidans KUESCCHK-6, Pseudomonas aeruginosa KUESCCHK-5, Achromobacter insuavis KUESCCHK-7 and Brevibacillus panacihumi KUESCCHK-8 with accession numbers OM475749, OM475764, OL797980 and OM475766 respectively. Bacteria can survive in contaminated environments because of their metabolic ability to use available resources and ability to find a suitable niche.