Abstract
11-Keto-17-hydroxypregnanolone was isolated from the urine of patients with congenital virilizing adrenal hyperplasia. The identification was based on: its mobility on paper chromatograms and the pink spot obtained when treated with the Zimmermann reagent; the product of oxidation with sodium bismuthate had an Rf value and a color reaction with the Zimmermann reagent similar to that of 11-ketoetiocholanolone; its ultraviolet spectrum as a sulfuric acid chromogen, and its infrared spectrum. A method for the measurement of this steroid based on its ltravioletabsorption as a sulfuric acid chromogen is described. In the simple virilizing form, patients of 5 to 39 years of age excreted 1.1–4.9 mg/24 hr of 11-keto-17-hydroxypregnanolone, while 2 infants under 1 year excreted 0.8 and 0.9 mg. In the salt-losing form, 5 patients under 1 year of age excreted 0.3–0.8 mg/24 hr. In subjects with the hypertensive form this steroid could not be detected.
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