Isolation, Identification and Inducing Systemic Resistance to Beet Mosaic Virus (BTMV) Infecting Sugar Beet Plants in Egypt

Abstract

The objective of the study described in this paper was to isolate and determine  a range of a potyvirus in sugar beet crop. The virus inoculum was taken from obviously diseased sugar beet plants (Beta vulgaris L.cv. Top), screening mild to severe mosaic complemented with leaves malformation and distortion, suspected to be caused by virus infection. Leaf samples were togethered from different areas in Menoufia Governorate, Egypt. The determination of the isolate was based on symptomatology, host range, modes of transmission (mechanical- insect), serological tests (Direct ELISA, DBIA and TBIA ), light microscopy. BtMV infection distributed some of photosynthetic pigments in infected beet leaves, i.e. chlorophyll, carotenoids and also reduced the concentration of total carbohydrates and total sugar in roots. Currently, different inducers stemmed from several materials were used to decrease the virus concentration. Preliminary showing were actual in reducing the number of local lesions formed by challenge inoculation of BtMV. Both inducers ( plant extracts and Salicylic acid (SA) showed reducing in the total numbers of local lesions and concentration of the virus. Among the inducers used for introduction of resistance against BtMV, Dianthus caryophyllus extract was found to be most effective. Also, Our results revealed that Salicylic acid (SA) used for screening  was effective in reducing the concentration of virus  and the number of local lesions formed by the challenge inoculation of BtMV .