Abstract
Purpose: To develop a method to identify and quantify the compounds in the shoots of four Phyllostachys bamboo species ( Gramineae Phyllostachys prominens W. Y. Xiong, Gramineae Phyllostachys iridescins C. Y. Yao Gramineae Phyllostachys pubescens (Carr.) Mitford, Gramineae Phyllostachys praecox C. D. Chu et C. S. Chao. ). Methods: The compounds in bamboo shoots were isolated and identified by ultraviolet (UV) spectroscopy, mass spectrometry (MS), and nuclear magnetic resonance (NMR). Quantitative analysis was performed by reversed-phase high performance liquid chromatography (RP-HPLC) using a C18 column and a mixture (1:1ratio) of acetonitrile and 15 mM ammonium acetate (pH 6.0) as mobile phase. This method was validated for its reproducibility, chemical stability, and recovery. Results: Six nucleosides and two amino acids were isolated from bamboo shoots, including guanosine, 2’-deoxyguanosine, adenosine, thymidine, uridine, cytidine, tryptophan, and phenylalanine. The HPLC method was rapid and reproducible. The intraday and interday concentrations of the eight identified compounds showed good linearity in the range of 0.22 - 60.00 μg/mL. The relative standard deviation (RSD) for intraday and interday precision for reproducibility and stability was < 3 %. The validated method was successfully applied to determine the content of the eight compounds in four different Phyllostachys species. Conclusion: Adenosine was isolated from bamboo shoots previously, but the isolation of the other seven compounds are reported here for the first time. The method proposed is sensitive and reproducible, and would facilitate studies of nutritional/medicinal compounds in bamboo shoot. Keywords: Bamboo shoots, Phyllostachys prominens , Guanosine, 2’ Deoxyguanosine, Adenosine, Thymidine, Uridine, Cytidine, Tryptophan, Phenylalanine
Highlights
Bamboo shoot is an important traditional food and medicinal material
The mobile phase consisted of Solvent A and Solvent B (15 mM ammonium acetate in water, pH 6.0)
The current study developed a method for the extraction, isolation, and identification of six nucleosides and two amino acids from bamboo shoots
Summary
Bamboo shoot is an important traditional food and medicinal material. The Compendium of Materia Medica in China records many medical uses of bamboo shoot. The chemical composition and active properties of bamboo shoots are attracting the attention of health advocates and scientists [1]. Bamboo shoot has a low fat content and is a rich source of nutrients such as vitamins, amino acids, and antioxidants such as flavones, phenols, and steroids [2]. Bamboo shoot is reported to possess anti-aging, antioxidant, anticancer, antibacterial, and antiviral activities [3]. Few studies have been performed on bamboo shoots of Phyllostachys prominens, which is an important crop species for bamboo shoots [4]
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