Isolation, Identification and Degradation Characteristics of Acetochlor Degradation Bacterium WN-3

Abstract

A bacterium named WN-3 which was able to degrade acetochlor, was isolated from the soil of long term applied with acetochlor by enrichment culture. Based on physiological and biochemical characteristics and 16 S r DNA sequence analysis, the strain WN-3 was identified preliminarily as Burkholderia sp.. The effect of culture time, temperature, initial p H, inoculation amount and concentration of acetochlor on growth and degradation efficiencies were studied. The results showed that the degrading efficiency could reach 38.8% under the optimal con-ditions of initial p H 6.0, inoculation amount of 10%, concentration of acetochlor 50 mg·L-1, respectively, after 7 days at 35 °C. It provided theoretical basis for using a bacteria of Burkholderia sp. in situbioremediation of soil pollution of acetochlor.