Isolation, identification and chemical characterization of compounds from phenolic extracts of peels of Kufri Chipsona-3 and Kufri Jyoti potatoes having synergistic antioxidant interactions in combination

Abstract

The study focused on the isolation, identification and characterization of the bioactive compounds that are responsible for synergistic antioxidant interactions in combination from the peel extract of Kufri Chipsona-3 and Kufri Jyoti potato varieties by Thin-Layer Chromatography (TLC) bioautography mediated isolation technique which was followed by isobologram analysis as well as characterization using different spectrometric methods. The results revealed that among the isolated seven antioxidant components of potato peel extracts (three components with Rf : 0.33, 0.52 and 0.57 from the peel extract of Kufri Chipsona-3 and four components with Rf : 0.14, 0.22, 0.36, 0.61 from the peel extract of Kufri Jyoti, only one component from Kufri Chipsona-3 (Rf : 0.57) and one component from Kufri Jyoti (Rf : 0.61) potato varieties in combination exhibited synergistic antioxidant interactions in combination. Spectrometric analysis (UV/Vis, FT-IR and HR-LCMS/MS) revealed that the active component with Rf : 0.57 from Kufri Chipsona-3 is prodelphinidin trimer and the other component with Rf : 0.61 from Kufri Jyoti is 5-hydroxy-3’,4’,7-trimethoxyflavone. In a root growth inhibition test (LC50 > 200 µg/ml), on Allium cepa, these two compounds showed no cytotoxicity. The results provide evidence that these two phenolic compounds, prodelphinidin trimer and 5-hydroxy-3’,4’,7-trimethoxyflavone in combination may serve as a stronger and efficacious novel natural antioxidant blend in pharmaceutical and food industries.