Abstract
A novel, lipid‐degrading bacterium (strain AHD‐1) was isolated from soil regularly contaminated with washing‐machine wastewater in Sfax, Tunisia. When this strain was grown in a medium containing 2% triacylglycerol, the hydrolysis products were found to be diacylglycerols, monoacylglycerols and free fatty acids. This strain was an aerobic, mesophilic, Gram‐negative, motile, non‐sporulating bacterium, capable of growing optimally at pH 7 and 27 °C. The predominant fatty acids were found to be C16:1ω7c (31%), C16:0 (28.1%), C18:1ω7c (16.3%) and C17:0 (5.8%). Phylogenetic analysis of the 16S rRNA gene showed that this isolate is a new strain belonging to the genus Pseudomonas. Strain AHD‐1 was found to be closely related to Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T with 99.7%, 99.56% and 99.54% of similarity, respectively.
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