Abstract

Some highly efficient diesel oil-degrading bacteria were isolated from bilge water. One isolated strain C7 was identified as Pseudomonas sp. based on its 16S rDNA gene sequence as well as various morphological, physiological and phylogenetic characteristics. The biodegradation potential and characteristics of strain C7 in various conditions were analyzed. GC-MS analysis showed that this strain could degrade most components of diesel oil, and the degradation rate was up to 75.9% after ten days incubation at the optimal condition which included a temperature of 30°C, a pH level of 7.0, initial diesel oil concentrations of 3% (v/v) and initial bacteria concentrations of 4 × 107cells/ml. Key word: Bilge water, biodegradation, diesel oil and Pseudomonas sp.

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