Abstract
Hydrilla verticillata contains some active compounds that potential as an antioxidant, antibacterial, anticancer, antimicrobial and antitumor. One of the active compounds in Hydrilla verticillata is steroids. This research aimed to isolate, to identify and to determine the toxicity and antioxidant activity of steroid compounds in petroleum ether (PE) fraction of Hydrilla verticillata. Hydrilla verticillata biomass powder was extracted by maceration using ethanol solvent. The ethanol extract was hydrolyzed with 2 N of hydrochloric acid and then partitioned with petroleum ether solvent. The steroid compounds from petroleum ether fraction were separated with Preparative Thin Layer Chromatography (TLC) and Column Chromatography. The steroid isolates were identified by UV-Vis and FTIR spectrophotometer. The toxicity level and antioxidant assay of steroid isolates were determined by BSLT and DPPH method, respectively. The result of the study showed that extraction through maceration produced 4.54% yield, whereas the product yield of the partition using petroleum ether was 65.41%. The steroid isolates from TLC and Column Chromatography separation has toxicity and antioxidant properties. The LC50 value of steroid TLC isolate was 1.41 and 12.2 ppm. The LC5o value of steroid Column Chromatography isolates (H2, H4 and H10) were 4.32, 8.24 and 10.35 ppm. The EC50 value of H2 Column Chromatography isolate was 23.00 ppm.
Highlights
Hydrilla verticillata are aquatic plants that are often found in Indonesian waters [1]
The steroid compounds from petroleum ether fraction were separated with Preparative Thin Layer Chromatography (TLC) and Column Chromatography
Materials The material used in this study was H. verticillata from Ranu Lake Pasuruan were n-hexane 96%, sulfuric acid 98%, chloroform 96%, ethanol 95%, HCl 2 N, saturated Na-bicarbonate, ethyl acetate p.a, acetic anhydride p.a, ethanol p.a. dimethyl sulfoxide, bread yeast, seawater, silica gel F254 plate, silica gel 60 (0.063-0.200 mm), DPPH, and Artemia salina L. eggs
Summary
Hydrilla verticillata are aquatic plants that are often found in Indonesian waters [1]. This plant is found in rivers, reservoirs and lakes [2]. The H. verticillata has various antioxidant and antimicrobial activities [5], antitumor [2], antimalarial [6], antiaging, detoxification agent [7], and anti-inflammatory [8]. These diverse activities are due to the presence of secondary metabolites in H. verticillata. The secondary metabolite compounds contained in H. verticillata include alkaloids, flavonoids, saponins, triterpenoids and steroids [9]
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