Abstract
A cross – sectional study was conducted from November 2016 to May 2017 in Dairy cattle in and around Asossa town in order to estimate the prevalence of mastitis, to identify S. aurues from mastitic lactating cows, to evaluate its antimicrobial resistance pattern and to identify risk factors associated with mastitis. A total of 384 Dairy cows milk samples were collected using purposive sampling techniques. The overall prevalence of mastitis at cow level was 39.32 % with 11.45 % and 27.86 % of clinical and subclinical mastitis, respectively. In this study, the subclinical mastitis was significantly higher than clinical mastitis. For all except Age and parity, the multivariable logistic regression analysis for intrinsic and extrinsic risk factors showed significant difference in the prevalence of mastitis in the study area (P˂0.05). From 151 mastitis infected lactating cows, 436 milk samples were cultured and 22.14% S.aureus were isolated. Presumptively identified S. aureus isolates were subjected to antimicrobial susceptibility test and 63(74.11%) MRSA have been identified from a total of 85 S. aureus using cefoxitin through disk diffusion method. The present result showed a significant association of resistance pattern with S.aureus isolates, particularly to penicillin G (95.55%), Cefoxitin (77.19%), Tetracycline (63.41%), Streptomycin (60.78%), Gentamycin (59.37%), Vancomycin (56.75%), Clindamycine (54.35%) and Bacitracin (53.65%). In this study, 77.19 % S.aureus isolates were found resistant against Cefoxitin. There were also observed multidrug resistance, mainly to Penicillin G, Streptomycin and Tetracycline. The present study revealed higher prevalence of mastitis and occurrence of multidrug resistance S.aureus specifically which belongs to the MRSA which are dependent on multiple associated risk factors. Hence, regular resistance follow-up, using antimicrobials sensitivity tests helps to select effective antimicrobials and to reduce the problems of drug resistance developments towards commonly used antimicrobials.
Highlights
2017 in Dairy cattle in and around Asossa town in order to estimate al susceptibility, Dairy cattle, milk, the prevalence of mastitis, to identify S. aurues from mastitic lactating cows, to evaluate its antimicrobial resistance pattern and to identify mastitis, Methicillin resistance, risk factors associated with mastitis
How to cite this article: identified S. aureus isolates were subjected to antimicrobial suscep- Asmamaw Aki et al, Isolation, tibility test and 63(74.11%) MRSA have been identified from a total Identification and Antimicrobial reof 85 S. aureus using cefoxitin through disk diffusion method
The present result showed a significant association of resistance pattern sistance profile of staphylococcus with S.aureus isolates, to penicillin G (95.55%), Cefoxitin aureus and occurrence of methi
Summary
Ethiopia has the largest cattle population in in companion and food animals and in their. Mastitis is among the Few studies have been carried out in some various factors contributing to reduced milk areas of Ethiopia to assess the status of production [7]. The pathogen of intramammary infections in dairy objectives of the present study were to cattle It is an important opportunistic pathogen determine the prevalence of bovine mastitis, to both in humans and in dairy cattle [14,37]. S. aureus is present in a variety of locations in lactating cows, to assess the risk factors the dairy farms, in many occasions it was associated with Staphylococcus infections and isolated from swabs taken from the cows head, to determine antimicrobial resistance pattern of skin swabs, legs and nasal mucosa and on S. aureus species the milkers’ hands. An infected udder quarter remains the main reservoir of the Materials and methods bacteria, which transmitted mostly during the Study Area milking time [17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
