Isolation, Identification and Antimicrobial Resistance Profile of Pathogenic Staphylococcus Aureus from Intensive Dairy Farms in Modjo and Adama, Central Ethiopia

Abstract

Staphylococcus aureus plays its most significant animal pathogenic role as cause of intra mammary infections in cattle and small ruminants leading to considerable economic losses in cattle farming. A cross sectional study was conducted from February to June 2019 in Adama and Modjo town, to isolate and identify S. aureus and their susceptible to different antimicrobials. Out of a total of 160 samples examined,20.6 % (33) were positive for S. aureus. Of this, 13/86 (15.1%), 20/74(27%) were positive for S. aureus atModjo and Adama, respectively. All 33 isolates were tested for antimicrobial resistance with 12 selected antibiotics. The isolates were highly susceptible to gentamycin (100%), streptomycin 24(72.7%), ciprofloxacin21 (63.6)), and kanamycine20 (60.6%), however, they were highly resistant to methicillin 31(93.9%), penicillin G 30(90.9%), tetracycline 21 (63.6%) and erythromycin (60.6%) respectively. Out of the resistance S. aureus isolates, 31 (93.9%) were developed multidrug resistant against 8 antibiotics. The S. aureus isolates from the dairy farms in the study area were multi drug resistant. Hence, public awareness about good management in dairy farms and milk handling practices, rational use of drugs and periodic assessment of drug sensitivity test should be done to prevent the current global issue which is antimicrobial resistance.