Isolation, identification and antibiogram profiles of Staphylococcus aureus from commercial broiler flocks in Dinajpur District of Bangladesh with special focus on the determination of lethal effect of extracted toxin

Abstract

The present study was designed with a view to isolate, identifies and characterizes Staphylococcus aureus from commercial broiler flocks having typical symptoms in Dinajpur District of Bangladesh with special focus on the determination of lethal effect of extracted toxin in day old chicks. The samples (pus/fluid) were subjected to bacterial isolation and identification by using cultural and biochemical techniques. Furthermore, the isolated Salmonella species were characterized by antimicrobial susceptibility testing. The study shown that the highest percentage (52.17%) of Staphylococcus aureus was obtained from group A followed by group B (41.67%) and group C (20.0%). Prevalence of toxicity in case of day old chicks those were given toxin orally was 30% and those were inoculated subcutaneously was 10%. Out of 30 day old chicks 04 died and average prevalence of toxicity is 20%. In postmortem examination it was found that tenosynovitis, most commonly in the plantar area of the foot or just above the hock joint. This was progress to abscess formation in these areas. Infected joints had clear exudate with fibrin clots. The abdomen felt soft, mushy, flabby and enlarged. Lesions associated with heavy challenge of infection generally consist of congestion of internal organs, including the liver, spleen, kidneys, and lungs, accompanied by areas of tissue death. The isolates were resistant to cephradine, ampicillin, bacitracin, amoxacillin and sulphamethoxazole-trimethoprim. It was evident that ciprofloxacin, nitrofurantion, gentamycin and penicillin can be of better value in the treatment of Staphylococcus aureus infection.