Isolation, identification, and activity evaluation of diterpenoid alkaloids from Aconitum sinomontanum

Abstract

A phytochemical study led to the isolation of 25 diterpenoid alkaloids from Aconitum sinomontanum, of which six were described for the first time. Among them compounds 1–3 are anhydrolycoctonine derivatives, rare rearranged aconitine-type C19-diterpenoid alkaloids. To our best knowledge, less than ten of this type of alkaloids were isolated just from the genus Aconitum. The structures of these unreported compounds were elucidated by extensive analysis of NMR spectroscopic data and X-ray diffraction. The biological activities of compounds 1–3, 5–9, and 12–25 were evaluated. Among the tested compounds, compounds 2 and 17 showed potent inhibitory effect on the capsaicin (selective TRPV1 agonist) mediated activation of transient receptor potential vanilloid 1 (TRPV1) channels expressed in HEK-293 cells with inhibition rate of 31.78% and 30.94% at the concentration of 10 μM. Compounds 1–3, 5–9, 13, and 18–25 exhibited weak cytotoxic activity against human tumor cell lines NCI–H226 and MDA-MB-231 with inhibition rate over 10% at the concentration of 10 μM. Compound 16 showed most inhibitory effect on the expression of Nrf2 (NF–E2-related factor-2)-regulated gene with inhibition rate of 25% at the concentration of 20 μM.