Abstract
Lycium minutifolium J. Remy (Solanaceae) is commonly used as an infusion in traditional medicine to treat stomach pain, meteorism, intestinal disorders, stomach ailments, and other severe problems including prostate cancer and stomach cancer. From the EtOAc extract of L. minutifolium bark five known metabolites were isolated using chromatographic techniques. The gastroprotective effects of the EtOAc fraction and edible infusion extract of the bark were assayed on the hydrochloric acid (HCl)/EtOH induced gastric ulcer model in mice to support the traditional use of the plant. The EtOAc extract and the edible infusion showed gastroprotective effect at dose of 100 mg/kg reducing lesions by 31% and 64%, respectively. The gastroprotective action mechanisms of the edible infusion at a single oral dose of 100 mg/kg were evaluated suggesting that prostaglandins, sulfhydryl groups, and nitric oxide are involved in the mode of gastroprotective action. The UHPLC analysis coupled to high-resolution mass spectrometry of the edible infusion showed the presence of twenty-three compounds. Our results can support the gastroprotective properties of the edible infusion extract, and at least can validate in part, the ethnopharmacological uses of the plant.
Highlights
Peptic ulcer disease refers to a group of ulcerative disorders that occur only in those parts of the digestive tract exposed to acid and pepsin produced by gastric mucosa
The bark of L. minutifolium was macerated with EtOAc for nine days at room temperature
Ten milligrams of the edible L. minutifolium aqueous extract (EI-EXT) was dissolved in fresh water, filtered and injected in the UHPLC-MS/MS machine (Figure 3 and Table 1). This infusion was chosen for metabolomic profiling due to the higher biological activity previously shown in comparison to that of ethyl acetate extract (EA-EXT) on the Hydrochloric acid (HCl)/EtOH-induced gastric lesions model in mice
Summary
Peptic ulcer disease refers to a group of ulcerative disorders that occur only in those parts of the digestive tract exposed to acid and pepsin produced by gastric mucosa. Peptic ulcer disease is generally thought to be a breakdown in the balance between two opposing forces: the aggressive luminal factors and the gastric mucosal barrier. Hydrochloric acid (HCl) secreted by oxyntic cells and pepsin produced by chief cells in the gastric mucosa are well-known aggressive factors [1]. Among exogenous factors implicated in the pathogenesis of peptic ulcers, ethanol, steroid drugs, smoking, stress, non-steroidal anti-inflammatory drugs, genetic influences, viruses and bacteria such as Helicobacter pylori can be mentioned [1]. H. pylori has been implicated as the main agent of gastric cancer since it is related to chronic gastritis. An alternative for prevention of this condition is the use of combined strategies for the prevention of peptic ulcers with the use of natural gastroprotective agents
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