Isolation Endophytic Bacteria of Mangosteen (Garcinia mangostana L.) Skin Fruit as of Escherichia coli Antibacterial

Abstract

Infectious diseases are caused by bacteria and fungi in developing countries are still high. One of the microorganisms that cause infections are Escherichia coli. E. coli can lead to infection of the digestive tract. Improper use of antibiotics can lead to resistance. This can be minimized by making use of traditional medicine. One of the traditional medicine that the mangosteen fruit. Secondary metabolites can be isolated by utilizing endophytic bacteria. Endophytic bacteria are bacteria that live inside plant tissues and capable of producing the active compounds which are antibiotics, antimalarial and antifungal. The methodology carried out: preparation of materials and determination, isolation of endophytic bacteria, screening endophytic bacteria producing antibacterial, morphological and biochemical identification, endophytic bacteria fermentation, the manufacture of standard curve, the compound fermented inspection (inspection: phenols, saponins, flavonoids, alkaloids and terpenoids/steroid), test the antimicrobial activity microdilution method. The result of morphological and biochemical tests showed that bacteria EKM 1 and EKM 2 is a Gram negative, motile, facultative anaerobic, produces gelatinase and protease enzymes. Antibacterial activity test microdilution method showed the presence of bacterial growth inhibition test. The endophytic bacteria isolated from mangosteen rind suspected Enterobacter. Antibacterial test results using microdilution method produces the MIC value 1024 ppm against E. coli bacteria.