ISOLATION, DEVELOPMENT AND VALIDATION OF QUERCETIN FROM THE ALLIUM ASCALONICUM’S TUNIC BY HPLC METHOD

Abstract

Allium ascalonicum, the tunic of which holds various bioactive compounds including including quercetin - a potent flavonoid, is renowned for its medicinal properties. This study presents the development and validation of a High-Performance Liquid Chromatography (HPLC) method for quantifying quercetin in Allium ascalonicum tunic. Validation of the method was conducted following International Conference on Harmonization (ICH) guidelines, evaluating parameters such as linearity, precision, and accuracy. This validated HPLC method offers an efficient and reliable means of quantifying quercetin in Allium ascalonicum tunic. Its application holds promise for routine analysis in pharmaceutical and nutraceutical industries, offering insights into the medicinal potential of Allium ascalonicum peels as a natural source of quercetin.