Isolation, Characterization, Determination of Probiotic Properties of Lactic Acid Bacteria from Human Milk

Abstract

s: Probiotic mean live microorganisms that have beneficial effects on their host's health. Although probiotic strains can be isolated from many sources; for human applications the main criteria is being human origin. Breast milk is an important nutrient source for neonates. Lots of studies showed that this fluid has beneficial effects on the health of neonates. One reason of being beneficial is explaining by the micro flora of human breast milk including beneficial lactic acid bacteria. In this study, isolates were identified by biochemical and molecular characterization and also probiotic properties of lactic acid bacteria, isolated from human milk were investigated. Two of the isolates were observed as potential probiotic. Two of them are bacilli. These isolates showed resistance to stomach pH (pH 3.0), tolerance against 0,3% bile concentration and antimicrobial activity against, Escherichia coli, Bacillus ceruse and Staphylococcus aureus and. After investigation the probiotic properties of these isolates, they were identified by biochemical characterization techniques and molecular identification by using Polymeric chain Reaction (PCR) of 16S ribosomal RNA (rRNA) and 16S sequencing. Two lactobacilli were identified as Lactobacillus rhamnosus (KF477283) and Lactobacillus casei. (KF477282) In the light of this study, it is observed that, human milk is a source of potential probiotic strains.