Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.).

Ramadoss Karthikeyan,Puttagunta Srinivasa Babu,Chapala Devadasu

doi:10.1155/2015/201386

Ramadoss Karthikeyan, Puttagunta Srinivasa Babu + Show 1 more

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https://doi.org/10.1155/2015/201386

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Abstract

P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn. (Pers.). In this study isolation of P-coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H1 NMR spectral analysis. An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass. The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5 μ), Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v). The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm. Retention time of P-coumaric acid was found to be 6.617 min. This method has obeyed linearity over the concentration range of 2–10 μg/mL and the regression coefficient obtained from linearity plot for P-coumaric acid was found to be 0.999. RP-HPLC method was validated in pursuance of ICH guidelines.

Highlights

It has been estimated that there are approximately 8,000 naturally occurring phenolic compounds
P-coumaric acid can be found in Gnetum cleistostachyum [9]
In food Pcoumaric acid can be found in a wide variety of edible plants such as peanuts, navy beans, tomatoes, carrots, and garlic. It is found in wine and vinegar [10]. It is found in barley grain [11]

Summary

Introduction

It has been estimated that there are approximately 8,000 naturally occurring phenolic compounds. Phenolic antioxidants are believed to provide a protective effect against oxidative damage diseases such as cancer, coronary heart disease, and stroke [1,2,3,4] and it has antidiabetic action [5, 6]. In food Pcoumaric acid can be found in a wide variety of edible plants such as peanuts, navy beans, tomatoes, carrots, and garlic. It is found in wine and vinegar [10]. P-coumaric acid has antioxidant properties and is believed to reduce the risk of stomach cancer [12], by reducing the formation of carcinogenic nitrosamines [13]

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