Isolation, Characterization and Antimicrobial Resistance Patterns of Lactose-Fermenter Enterobacteriaceae Isolates from Clinical and Environmental Samples

Israa Abdul Jabbar Ibrahim,Tuqa Abdul Kareem Hameed

doi:10.4236/ojmm.2015.54021

Abstract

The lactose-fermenter Enterobacteriaceae are the most frequent cause of clinical infection in our country. The objective of this study was to isolate and identify the most common lactose-fermenter Enterobacteriaceae from clinical samples, including urine, blood, wounds, and sputum, obtained from the local hospital and from environmental samples from a chicken farm, agriculture soil, and water from the Tigris River in Baghdad City. The study also aimed at establishing the antibiotic resistance patterns of the isolated bacteria. A total of 155 bacterial isolates were identified from 10 genera according to the Vitek 2 system. The most common bacterial isolates from the clinical and environmental samples were Escherichia coli and Klebsiella pneumoniae, respectively. The antibiotic resistance patterns showed that all clinical and environmental isolates were multidrug resistant to β-lactam (except carbapenems) drug and aminoglycosides and more sensitive to carbapenems.

Highlights

Enterobacteriaceae may account for 80% of clinically significant isolates of Gram-negative bacilli and 50% of clinically significant bacteria in clinical microbiology [4]
The present results showed the total viable count (TVC) of water was 3.26 × 104 cfu/ml (Table 1)
While Ibrahim et al report the highest TVC values were obtained in the Tigris study area from August and September, ranging from 128 to 10,000 cfu/ml [20]

Summary

Introduction

They are found in soil, water, fruits, meats, eggs, vegetables, I. Enterobacteriaceae are Gram-negative rods with a length of 1 - 3 μm. They are facultative anaerobes, oxidase-negative, catalase positive, and grow on MacConkey agar, and their natural hosts are human and animal intestines [2]. There are 44 genera and approximately 176 species [1]. Enterobacteriaceae may account for 80% of clinically significant isolates of Gram-negative bacilli and 50% of clinically significant bacteria in clinical microbiology [4]. Some Enterobacteriaceae species have only been identified based on their16S rDNA sequence and have not been isolated or characterized biochemically [1]

Objectives

Methods

Results

Conclusion