Isolation, Characterization and Antibacterial Activity of ECDY Steroid from Roots of Polycarpaea corymbosa LAM

Abstract

A new phytoecdysteroid (2- deoxy 20 hydroxy 22 cinnamoyl ecdysone 3, 25 diacetate) was isolated from the roots of Polycarpaea corymbosa Lam (family: caryophyllaceae).The powdered roots were extracted with petroleum ether ( 60-80? C), benzene, ethyl acetate and ethyl alcohol in soxhlet apparatus by simultaneous extraction. The highest yield of benzene extract was chosen for the isolation of ecdysteroid. The white solid pure compound was isolated and tested by TLC and respond positively for Liebermann - burchard reaction. Their structure has been established by UV, IR, 1H, 13C-NMR DEPT -90, DEPT -135 and EI- MS experiments. All the extracts and isolated compound were screened for anti-bacterial activity against B.subtilis, P.vulgaris, E.coli, P.aeruginosa by Kirbybauer method.