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Abstract
Black Soldier Fly (Hermetia illucens) larvae used as a large-scale organic waste bioreduction agent with ability reduce organic waste up to 200 tons per day. The potency of BSF larvae to process organic waste was related to the function of phisiology, microbial and biochemical processes. The gut extracts had high amylase, lipase and protease activities. α-amylase is one of the important enzyme in development of biotechnology and belongs to the main enzyme class on the world enzyme market. α-amylase used to replace chemical hydrolysis process in starch processing industry. α-amylase from microorganism was more prospective than plant and animal amylase, because it was more easily manipulated to produces targetted enzymes, characteristics and more economical. The purpose of this study was to isolate bacteria producing α-amylase from BSF larvae. The research method was extracted of 200 BSF larvae in phosphate buffer pH 7.4. Homogenate was isolated in Lysogeny Broth (LB) by serial dilution and fourway strick. Each culture was screened based on the activity of α-amylase by Fuwa method. The results of isolation obtained 8 single colonies bacteria. The α-amylase activity assay for Fuwa method obtained the highest α-amylase activity unit was 10.86 units/ml, produced by the 8th colony.
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