Isolation and Synthesis of Crosslinked Gelatin of Waste Lates Calcarifer Scales Using Gamma Irradiation Induction Technique

Abstract

In order to use the fisheries waste, isolation of gelatin has been carried out from Lates calcarifer scales and then synthesized to be cross-linked gelatin by induction of gamma irradiation at irradiation dose of 0-50 kGy. Chemical structures change of irradiated gelatin was characterized by using UV-Vis and FT-IR spectrophotometer. The viscosity of gelatin was measured by using viscosimeter. The results showed that radiation dose correlate linearly to absorbance and viscosity of gelatin solution at doses of 10-30 kGy. While at doses 40-50 kGy, gelatin solution was transformed from solution to gel with significant change in FT-IR spectrum. Based on this result, gamma irradiation technique should be considered as a safe and quick technique to synthesis cross-linked gelatin. Keywords: Fish scale, Gelatin, Gamma irradiation, Crosslinking.