Isolation and Studying the Effect of Proteinous Fractions of Unripe Banana Fruit (Musa paradisiaca L.) on Some Biochemical Parameters in Experimental Diabetic Rats

Abstract

The research was an attempt to isolate and study the active proteinous compounds from the aqueous extract of unripe banana (Musa paradisiaca L.), using different biochemical techniques. One major compound was isolated using gel filtration chromatography for the precipitate produced by ammonium sulphate treatment. The comparative molecular weight of the isolated proteinous compound was found to be 12566 Dalton. The study also included the effect of the aqueous extract and the proteinous compound in two doses (75, 100 mg/kg body weight) on certain blood constituents in normal and alloxan induced diabetic rats. In normal rats, the blood glucose level was significantly decreased by crude aqueous extract and proteinous compound in both doses (75, 100 mg/kg body weight) compared with control group. In addition, the proteinous compound in a dose ( 100 g/kg body weight) showed a significant decrease in serum cholesterol and total lipids level. In diabetic rats, the proteinous compound in a dose of (100mg/kg body weight) showed a significant decrease in serum glucose, cholesterol and total lipids levels. ــــــــــــــــ ــــــــــــــــ ــــــــــــــــ ـــــــــ