ISOLATION AND STUDY OF POLYSACCHARIDES OF MARGILAN RADISH RAPHANUS SATIVUS

Abstract

The purpose of this research is to isolate and study the physicochemical characteristics of polysaccharides in the seeds of the Margilan radish of the Brassicaceae family cultivated in the Republic of Uzbekistan.
 For the first time, a water-soluble fraction of polysaccharides was isolated from radish seeds by sowing Margilan successive extraction after the extraction of proteins and polyphenols. Anion exchange chromatography and gel filtration were used to obtain homogeneous polysaccharides. The polysaccharide fraction was separated by ion-exchange chromatography, purified on a Sephadex G-100 column. As a result, two polysaccharides RSP1 and RSP2 were obtained. The monosaccharide composition of the isolated polysaccharides was established. Analysis of monosaccharide residues showed that the composition of the RSP1 polysaccharide is represented by monosaccharides in the following composition: rhamnose - 0.7%, ribose - 4.7%, arabinose - 55.6%, xylose - 1.5%, mannose - 3.7%, glucose - 5.1% and galactose - 28.7%. The composition of the RSP2 polysaccharide is represented by monosaccharides: ribose - 2.8%, arabinose - 9.4%, mannose - 0.9%, glucose - 64.9% and galactose - 18.4%. From the obtained results, it can be seen that the RSP1 polysaccharide consists mainly of arabinose (55.6%) and galactose (28.7%) residues and belongs to the type of arabinogalactans, and the RSP2 polysaccharide mainly consists of glucose residues (64.9%) and galactose (18.4%).