Abstract
Rhizobactin 1021, a novel siderophore from the nitrogen-fixing alfalfa symbiont Rhixobium meliloti 1021, was isolated and structurally characterized by a combination of chemical and spectroscopic techniques. The compound is a citrate derivative in which the distal carboxyl groups of the molecule are amide linked to two different side chains. One of these is 1-amino-3-(N-hydroxy-N-acetylamino)propane, and the other is 1-amino-3-(N-hydroxy-N-(E)-2-decenylamino)propane. The structure of rhizobactin 1021 was determined as (E)-4-[[3-(acetylhydroxyamino)propyl]- amino]-2-hydroxy-2-[2-[3-[hydroxy(1 -oxo-2-decenyl)amino]propyl]amino]-2-oxoethyl]-4-oxobutanoic acid
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