Abstract
The present study was conducted to isolate and classify Dalbergia sissoo (L.) bioactive compounds. The genus consists of 300 species in India, including 25 species. The generic name Dalbergia honors the 18th century Swedish brothers Nils and Carl Dalberg. Various phytoconstituents of alkaloids, glycosides, flavanoids, tannins, saponins, sterols and terpenoids were isolated and classified from different parts of the plant. Thin Layer Chromatography, High Performance Thin Layer Chromatography, and Column Chromatography were used to isolate spots from the fraction of the crude extract to elucidate the chemical structure of Dalbergia sissoo (L.) leaf extract. Potential spots have been exposed to techniques of FTIR, NMR and mass spectroscopy. Column chromatography was exposed to the raw extract, obtaining 125 fractions, conducting TLC. Among them was a single band in TLC, characterized by FTIR, NMR spectroscopy and mass spectroscopy, and the structure was known as Quercetin. The results of this study indicate the effective potential compound of the ethanolic fraction of Dalbergia sissoo (L.).
 Keywords: Dalbergia sissoo; Isolation & Structure Elucidation; FTIR; NMR spectroscopy; Mass spectroscopy.
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