Isolation and structural analysis of microsomal membrane proteins.

Abstract

Investigation of the endoplasmic reticulum requires a methodological background in the solubilization, purification, and structural analysis of membrane proteins. The experience of one laboratory with microsomal proteins over 30 years is summarized in this review. We focus on the isolation and structure of the major proteins of rabbit liver microsomes. The special pitfalls encountered with fragile protein complexes, hydrophobic peptides, and post-translational modifications are emphasized.