Abstract
An obligate and a facultative strains of iron-oxidizing bacterium, Thiobacillus ferrooxidans, were isolated and their properties were investigated. No difference was observed in the iron-oxidizing system of the two strains, while a distinct difference was observed in the D-glucose metabolizing system. Intact cells of the obligate strain, AP-19, did not have D-glucose oxidizing and 14C-glucose uptake activity, while they were both present in intact cells of iron grown, iron-glucose grown and glucose grown facultative strain, AP-44. Since cell-free extracts from both strains showed D-glucose oxidizing activity to the same degree, it was concluded that the obligate nature in AP-19 was due to lack of D-glucose uptake activity.
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