Abstract
A cDNA encoding Δ 1-pyrroline-5-carboxylate reductase (P5CR) was isolated from the pneumocandin(Pmo)-producing fungus, Zalerion arboricola (Za), by complementation of a P5CR-deficient mutant ( pro3) of Saccharomyces cerevisiae (Sc). The cloned cDNA was placed under control of the Sc galactokinase ( GAL1) promoter and restored P5CR activity to the pro3 mutant. Sequence analysis revealed that the Za P5CR-encoding cDNA encodes an approx. 35 kDa protein with substantial amino acid (aa) identity to P5CR from another filamentous fungus, Neurospora crassa (Nc). Za P5CR exhibits a moderate degree of aa identity to P5CR from plants, bacteria, human and Sc. This is the first gene to be isolated from Za.
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