Abstract
Microbial proteases account for approximately 40% of the total worldwide enzyme sales and most of the commercial proteases are of bacterial origin. Total 35 bacterial species isolated from soil and water collected from different districts of Gujarat. Isolates were screened on skim milk agar plates (pH-8.5) for their potency to secrete alkaline protease. Out of these, 14 cultures were screened based on relative enzyme activity (REA) on solid media. Cultures showing more than 3 REA were selected for further studies by using shake flask method. Bacillus spp. C6 was selected for this study and characterized by cultural, biochemical and microscopic features. All the data of the isolates that it may belong to Bacillus circulans.
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