Isolation and screening of phenol-degrading bacteria from pulp and paper mill effluent

Preeti Sachan,Sangeeta Madan,Athar Hussain

doi:10.1007/s13201-019-0994-9

Preeti Sachan, Sangeeta Madan + Show 1 more

Open Access

https://doi.org/10.1007/s13201-019-0994-9

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Abstract

Phenol and its derivatives are pollutants present in the effluents of major industries such as paper mill, oil refineries and petrochemical plants. Removal of phenol from industrial effluent is extremely important because of its toxicity to the aquatic life and environment. In the present study, an attempt has been made to eradicate the phenol from wastewater using isolated bacteria from chronically contaminated effluent samples of a paper mill industry. The pH value of the effluent has been observed to be 8.2. The presence of high concentration of phenol has been observed in the effluent samples. The total sixteen bacterial isolates as obtained were checked for growth on minimal salt medium amended with different concentrations of phenol by flask culture technique. In the present study, the two isolate species of SP-4 and SP-8 were found to be very tolerant to degrade a phenol concentration up to 1800 mg/L.

Highlights

With the rapid increase in population and hasty industrialization in past few decades, the industries have emerged to be critical for the humans and the environment
Odour problem may be due to the presence of high concentration of phenol and its derivatives generally found in pulp and paper mill effluents and are not biodegradable
Two bacterial strains SP-4 and SP-8 have been isolated from the pulp and paper mill effluent and screened out for phenol degradation

Summary

Introduction

With the rapid increase in population and hasty industrialization in past few decades, the industries have emerged to be critical for the humans and the environment. The effluents from these industries are proved to be the main source of numerous kinds of pollution in natural water. It is very important to remove phenol from contaminated water before discharge into any natural water because of their toxicity to aquatic organisms. It has severe effect on human being, both short and long term (Sonawane and Koreke 2016)

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