Isolation and screening of multifunctional rhizobacteria from the selected sites of Madhupur, Narshingdi and Mymensingh, Bangladesh

Atiqur Rahman,Md Shahinur Islam,Moonmoon Nahar Asha,Quazi Forhad Quadir

doi:10.3329/ralf.v2i1.23020

Abstract

A laboratory experiment was performed to isolate some native rhizobacteria that could be used as bioinoculants for sustainable crop production. A total of 43 rhizobacteria were isolated from undisturbed plant rhizosphere soils of three different locations of Bangladesh and evaluated their plant growth promoting traits, both direct and indirect. The study has screened out isolates on the basis of their phosphorous solubilization and nitrogen (N) fixation. The phosphate solubilization assay in National Botanical Research Institute of Phosphate (NBRIP) medium revealed that 12 bacterial isolates were able to solubilize tricalcium phosphate and the rhizobacteria M25 showed best performance with a PSI of 3.33 at 5 day. Exactly 47% (20 isolates) of the isolated rhizobacteria were able to grow in N-free Winogradskys medium, which is an indication of potential N2-fixers. Among the 20 potential N-fixers, 15 were able to grow within 24 hours of incubation indicating that they are more efficient in Nfixation. The present study successfully isolated and characterized 43 rhizobacteria. Some of these isolated rhizobacteria have potential plant growth promoting traits and are potential plant growth promoting rhizobacteria (PGPR) candidate. Considering all plant growth promoting traits, the isolate F37 was the best followed by M6. However, further experiments are needed to determine the effectiveness of these isolates under in vitro and different field conditions to understand the nature of interaction with the plant and environment.Res. Agric., Livest. Fish.2(1): 1-8, April 2015

Highlights

ISOLATION AND SCREENING OF MULTIFUNCTIONAL RHIZOBACTERIA FROM THE SELECTED SITES OF MADHUPUR, NARSHINGDI AND MYMENSINGH, BANGLADESH
The phosphate solubilization assay in National Botanical Research Institute of Phosphate (NBRIP) medium revealed that 12 bacterial isolates were able to solubilize tricalcium phosphate and the rhizobacteria M25 showed best performance with a PSI of 3.33 at 5 day
Focus has been on the use of plant growth promoting rhizobacteria (PGPR) as an alternative, environmentally friendly and effective strategy for plant control (Babalola and Glick, 2012; Patel et al, 2012)

Summary

Introduction

ISOLATION AND SCREENING OF MULTIFUNCTIONAL RHIZOBACTERIA FROM THE SELECTED SITES OF MADHUPUR, NARSHINGDI AND MYMENSINGH, BANGLADESH. A total of 43 rhizobacteria were isolated from undisturbed plant rhizosphere soils of three different locations of Bangladesh and evaluated their plant growth promoting traits, both direct and indirect. PGPR are among the most complex, diverse, and important assemblages in the biosphere (Khan, 2005) They are considered as a group of beneficial free living soil bacteria for sustainable agriculture and environment (Babalola, 2010). PGPR are characterized by a number of activities, which include the capacity to colonize plant roots surfaces closely adhering to soil interface, increase mineral nutrient solubilization (i.e. P) and N- fixation (Khan, 2005; Shanab et al, 2003) promote plant growth and yield, suppress plant diseases and soil borne pathogens by the production of hydrogen cyanide (HCN), siderophores, antibiotics, and/or competition for nutrients (Kamnev and Lelie, 2000; Shanab et al, 2003; Idris et al, 2007). PGPR improve plant stress tolerance especially to drought, salinity, metal toxicity and production of phytohormones such as indole3-acetic acid (IAA) (Khan et al, 2009; Verma et al, 2010; Figueiredo et al, 2010)

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Conclusion