Abstract
Fungal colonies capable of tolerating and degrading endosulfan were enriched and isolated from soil with history of endosulfan application. The microorganisms were enriched using technical-grade endosulfan with gradual increase in concentration from 10 to 100 mgl-1, each enrichment run lasting for 210 h. Four (4) fungal isolates, labeled as P701, P702, P601 and P801, were obtained by successive plating of enriched colonies. The isolates were screened based on α- and β-endosulfan degradation in nutrient media with endosulfan as sole-carbon source (NM-SCS) and as sole-sulfur source (NM-SSS). Results indicated that P601 grown in the NM-SSS exhibited the highest endosulfan degradation after 14 d (93.85% for α-endosulfan and 90.29% for β-endosulfan). Isolate P601 was identified as Candida tropicalis using the API C AUX yeast identification system.
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