Isolation and regeneration of protoplasts from Solanum nigrum L.

Abstract

Leaf mesophyll protoplasts from sterile shoot cultures of Solanum nigrum were isolated by means of enzymic degradation of the cell walls at a yield of 5·10 5 to 10 6/g fresh weight. No viable protoplasts could be obtained from greenhouse plants. Protoplast division and cell cluster formation occurred in modified KM and in V-KM media. Subsequent culture on various solidified media revealed callus proliferation, organogenesis, and regeneration of whole plants.