Abstract
Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties. Sinigrin and gluconapin are the main glucosinolates present in brown mustard seeds (Brassica juncea). These glucosinolates are very suitable for the development of phytosanitary products due to their fungicidal, bactericidal and insecticidal effects. In this work, the purification of sinigrin and gluconapin extracted from defatted mustard seeds was studied using macroporous anion exchange resins. A strongly and a weakly anionic resin were first tested according to the nature of their functional group and through their selectivity towards glucosinolates. Anion-exchange resin purification was first studied in static (batch) mode in order to determine the optimal operating conditions; it was then tested in a dynamic (continuous) mode (column) to validate the process. In static mode, the adsorption behavior and characteristics of both resins were compared. The results showed that the strongly basic resin PA312LOH ensures better adsorption of glucosinolates and that the experimental data fit well with the Freundlich isotherm. Moreover, analysis showed that PA312LOH resin was selective for glucosinolates purification towards the proteins. The desorption of glucosinolates was then investigated. Firstly, the operating conditions were optimized by studying the effects of salt concentration and the eluate-resin ratio. This preliminary optimization allowed recovering 72.9% of intact sinigrin and the juice purity was increased from 43.05% to 79.63%. Secondly, dynamic (continuous mode) experiments allowed the recovery of 64.5% of sinigrin and 28% of gluconapin by varying the eluent ionic strength and the flow rate. Resin was finally successfully regenerated using NaOH.
Highlights
Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties
83.2% of gluconapin was adsorbed using strongly basic resin PA312LOH at the concentration 20 g/L, while only 72.9% of sinigrin was adsorbed in the same conditions
Preliminary showed that strongly basic resin PA312LOH has better affinity towards glucosinolates than showed that strongly basic resin PA312LOH has better affinity towards glucosinolates the RELITE RAM1 weakly resin
Summary
Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties. These molecules are inactive in their native form but they are precursors of various highly reactive products presenting fungicidal, bactericidal and insecticidal properties [1,2,3,4,5,6]. Sinigrin and gluconapin are the major GSL in mustard seeds (Figure 1) They are inactive unless interacting with an endogenous enzyme called myrosinase (ß-thioglucosidase, E.C. 3.2.1.147) to produce allyl and but-3-enyl isothiocyanates (AITC & BITC) respectively [9,10,11]. The isolation and purification of glucosinolates have been documented with different chromatography methods
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