Abstract
Materials and methods We developed a protocol using an ATP dependent plasmid safe DNAse that selectively digests linear DNA to purify LTR circles from chromosomal DNA. The β-actin gene was amplified to detect contamination from linear DNA. A phylogenetic analysis of the env HIV regions of the LTR circles, PBMCs and free virus in plasma from three patients undergoing HAART within 27 to 852 viral copies/ ml viral load range was undertaken to determine if LTR episomes are labile in vivo and suitable for analysis.
Highlights
The source of the HIV-1 virions that emerge during suppressive HAART can be identified by a phylogenetic analysis that compares the virions present in plasma with those in cell reservoirs
These findings support our hypothesis showing that episomal DNA is apparently reflecting the viral quasispecies present in plasma RNA, especially in the patient with the lowest viral load
We developed a protocol using an ATP dependent plasmid safe DNAse that selectively digests linear DNA to purify LTR circles from chromosomal DNA
Summary
Sandra González*, Sharilyn Almodóvar, Rafael A Contreras, Maria Colón, Martin D Hill and Eric Lorenzo. Address: Department of Biochemistry, Ponce School of Medicine, Ponce, Puerto Rico, 00732, USA * Corresponding author from 2006 International Meeting of The Institute of Human Virology Baltimore, USA. 17–21 November, 2006 Published: 21 December 2006 Retrovirology 2006, 3(Suppl 1):P20 doi:10.1186/1742-4690-3-S1-P20. 2006 International Meeting of The Institute of Human Virology Meeting abstracts. A single PDF containing all abstracts in this Supplement is available here http://www.biomedcentral.com/content/pdf/1742-4690-3-S1-info.pdf
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