Abstract
High-speed counter-current chromatography was used to separate and purify galloyl, caffeoyl, and hexahydroxydiphenoyl esters of glucoses from the aerial parts of the parasitic plant Balanophora simaoensis for the first time using n-hexane-ethyl acetate-methanol-water (1:2:1:2, v/v) as the optimum solvent system. Accordingly, 1-O-(E)-caffeoyl-3-O-galloyl-β-d-glucopyranose (I, 12.5 mg), 1-O-(E)-caffeoyl-3-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-β-d-glucopyranose (II, 27.2 mg), and 1-O-(E)-caffeoyl-4,6-(S)-hexahydroxydiphenoyl-β-d-glucopyranose (III, 52.8 mg) with 98.0%, 98.5%, and 98.7% purities, respectively, were purified from 210 mg crude extract of B. simaoensis in a one-step separation. The structures of the glucose esters were identified by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectra (NMR). Their antioxidant activities were evaluated by measuring their inhibition activity on liver microsomal lipid peroxidation induced by the Fe2+-Cys system in vitro. Compounds I–III showed significant antioxidant activities with IC50 values ranging from 2.51 to 6.68 μm, respectively.
Highlights
Balanophora simaoensis (Balanophoraceae) indigenous to China is a parasitic plant growing on the roots of various plants and mainly distributed in the Yunnan province, China [1]
In order to further study the biochemical properties of glucose esters from B. simaoensis and to evaluate their clinical applications, it is essential to develop an efficient method for the separation of glucose esters
The structures of three glucose esters were determined by electrospray ionization mass spectrometry (ESI-MS), 1 H-nuclear magnetic resonance spectra (NMR), and analysis
Summary
Balanophora simaoensis (Balanophoraceae) indigenous to China is a parasitic plant growing on the roots of various plants and mainly distributed in the Yunnan province, China [1]. In order to further study the biochemical properties of glucose esters from B. simaoensis and to evaluate their clinical applications, it is essential to develop an efficient method for the separation of glucose esters. Conventional methods for the isolation of these glucose esters are often based on the extensive use of multiple chromatographic steps, which is time-consuming and offered low recoveries of the target products [8,10]. Is apartition liquid–liquid partition chromatography, is capable eliminating the solid liquid–liquid carrier adsorption and offering totalcompounds. To the advantages and offering total recovery of target to the advantages Due of HSCCC, it has been widely used in and purification of separation different kinds natural products [11,12]. HSCCC method been preparative separation of glucose esters plants extracts using.
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