Abstract
The work included the isolation of Bromelain enzyme from pineapple juice using different biochemical techniques which included: ammonium sulfate precipitation, diagnosis and gel filtration chromatography on Sephadex G-100. It was found that only the second peak had a high activity for Bromelain, the apparent molecular weight of the isolated Bromelain using gel filtration chromatography and SDS-PAGE was (27000) and (24000) Dalton respectively, this study was led to distinguish the cytotoxic action of purified enzyme Bromelain from pineapple juice remove on human breast cancer (MCF-7) cells in vitro contrasted with human hepatic (WRL-68) cells utilizing MTT measure. Charted 6 crystal program incorporating non-straight relapse with one-way ANOVA followed by Dennett's different comparison test to get information addressing the mean+ SD. The outcomes uncovered high poisonousness of the concentrate against MCF-7 cells with IC50 (194.5µg/ml) and showed high-decreased action toward WRL-68 cells with high IC50 esteem while the least feasibility (26.389 ±3.73022) showed up in MCF. 7 cells under treatment with 400 µg.ml-1 contrasted and high practicality in WRL-68 cells. The review reasoned that has a significant wellspring of anticancer medications with protected and particular action on disease cells in contrast with human typical cells.
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