Abstract

A specific autoantigen of rabbit accessory glands was isolated and purified from seminal plasma using DEAE-Sephadex batch adsorption and Sephadex G-100 chromatographic fractionations. The autoantigen appeared homogeneous by polyacrylamide electrophoresis and by immunodiffusion. It has an extinction coefficient at 284 mμ (E 1cm 1%) of 6.42. It contains 87.73% of polypeptides. The carbohydrate moiety is composed of 9.21% hexose, 1.03% hexosamines, 0.33% fucose. From these chemical values the autoantigen has been identified as glycoprotein.

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