Abstract
In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys3 and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.
Highlights
Plants generate a variety of proteins that serve to protect against pathogens and invading organisms, include protease inhibitors [1] and antifungal proteins [2,3,4]
The extract of the potato tuber was fractionated on Sephacryl S-100 into unadsorbed fractions (Fraction I and Fraction II) without antifungal activity and an adsorbed fraction (Fraction III) with activity
The large absorbed peak was purified by two steps on a C18-reverse phase-high performance liquid chromatography (HPLC)
Summary
Plants generate a variety of proteins (peptides) that serve to protect against pathogens and invading organisms, include protease inhibitors [1] and antifungal proteins [2,3,4]. Plants have been shown to make several types of proteins (peptides) that mediate defense against pathogens and invading organisms, including ribosome-inactivating proteins [9], lectins [10], protease inhibitors [11,12], and antifungal proteins [13]. Among these proteins, protease inhibitors are believed to play an important role in the defense against attack by both microorganisms and insects. Potide-J was synthesized by solid-phase methods and its antifungal activity was evaluated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
