Abstract
AbstractTetrandrine is a bisbenzytehahydroisoquinoline alkaloid. Its structure is very complex and the preparation of labeled derivatives is difficult. We have prepared 3H‐tetrandrine using the tritium gas exposure method.1 This method produces many fragments which make isolation and purification of small quantities difficult. We investigated these fragments of unlabeled d‐tetrandrine and found almost all contain one to several hydroxide groups. Using this information, we designed a two‐step paper chromatography method for isolation and purification of 3H‐Tetrandrine. This method involves the application of a pH 4.7 Na2HPO4‐citric acid buffer to the paper to block the migration of hydroxide group fragments of tetrandrine. 3H‐tetrandrine was successfully with a radiochemical purity of 90%.
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