Abstract
Malus xiaojinensis Cheng et Jiang is a special and significant germplasm resource of semi-dwarf apple in China. Abiotic stresses, such as Fe and high salinity, affect Malus xiaojinensis growth and development. WRKY transcription factors (TFs) are widely involved in the responses of plants to different stresses. In the present study, a new WRKY gene was isolated from Malus xiaojinensis and designated as MxWRKY64. Subcellular localization showed that MxWRKY64 was a nucleus localized protein. The expression level of MxWRKY64 was highly affected by Fe and salt stress in M. xiaojinensis seedlings. When MxWRKY64 was introduced into Arabidopsis thaliana, it greatly increased the Fe and salt tolerance in transgenic plant. When dealt with Fe stress, overexpression of MxWRKY64 in transgenic A. thaliana contributed to higher levels of fresh weight, root length, and contents of chlorophyll and Fe than wild type (WT). Increased expression of MxWRKY64 in transgenic A. thaliana also resulted in higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), higher contents of proline and chlorophyll, while malondialdehyde (MDA) content was lower, especially in response to salt stress. Therefore, these results suggest that MxWRKY64 probably plays an important role in response to Fe and salt stress in Arabidopsis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: In Vitro Cellular & Developmental Biology - Plant
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
